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| Item Type: | Article |
|---|---|
| Title: | Non-SMC condensin I complex proteins control chromosome segregation and survival of proliferating cells in the zebrafish neural retina |
| Creators Name: | Seipold, S., Priller, F.C., Goldsmith, P., Harris, W.A., Baier, H. and Abdelilah-Seyfried, S. |
| Abstract: | BACKGROUND: The condensation of chromosomes and correct sister chromatid segregation during cell division is an essential feature of all proliferative cells. Structural maintenance of chromosomes (SMC) and non-SMC proteins form the condensin I complex and regulate chromosome condensation and segregation during mitosis. However, due to the lack of appropriate mutants, the function of the condensin I complex during vertebrate development has not been described. RESULTS: Here, we report the positional cloning and detailed characterization of retinal phenotypes of a zebrafish mutation at the cap-g locus. High resolution live imaging reveals that the progression of mitosis between prometa- to telophase is delayed and that sister chromatid segregation is impaired upon loss of CAP-G. CAP-G associates with chromosomes between prometa- and telophase of the cell cycle. Loss of the interaction partners CAP-H and CAP-D2 causes cytoplasmic mislocalization of CAP-G throughout mitosis. DNA content analysis reveals increased genomic imbalances upon loss of non-SMC condensin I subunits. Within the retina, loss of condensin I function causes increased rates of apoptosis among cells within the proliferative ciliary marginal zone (CMZ) whereas postmitotic retinal cells are viable. Inhibition of p53-mediated apoptosis partially rescues cell numbers in cap-g mutant retinae and allows normal layering of retinal cell types without alleviating their aberrant nuclear sizes. CONCLUSIONS: Our findings indicate that the condensin I complex is particularly important within rapidly amplifying progenitor cell populations to ensure faithful chromosome segregation. In contrast, differentiation of postmitotic retinal cells is not impaired upon polyploidization. |
| Keywords: | Adenosine Triphosphatases, Apoptosis, Cell Proliferation, Chromosome Segregation, DNA-Binding Proteins, Immunohistochemistry, In Situ Hybridization, Multiprotein Complexes, Retina, Tumor Suppressor Protein p53, Animals, Zebrafish |
| Source: | BMC Developmental Biology |
| ISSN: | 1471-213X |
| Publisher: | BioMed Central |
| Volume: | 9 |
| Number: | 1 |
| Page Range: | 40 |
| Date: | 8 July 2009 |
| Official Publication: | https://doi.org/10.1186/1471-213X-9-40 |
| PubMed: | View item in PubMed |
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