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| Item Type: | Article |
|---|---|
| Title: | 670 nm laser light and EGCG complementarily reduce amyloid-β aggregates in human neuroblastoma cells: basis for treatment of Alzheimer's disease? |
| Creators Name: | Sommer, A.P., Bieschke, J., Friedrich, R.P., Zhu, D., Wanker, E.E., Fecht, H.J., Mereles, D. and Hunstein, W. |
| Abstract: | Objective: The aim of the present study is to present the results of in vitro experiments with possible relevance in the treatment of Alzheimer's disease (AD). Background Data: Despite intensive research efforts, there is no treatment for AD. One root cause of AD is the extra- and intracellular deposition of amyloid-beta (A{beta}) fibrils in the brain. Recently, it was shown that extracellular A{beta} can enter brain cells, resulting in neurotoxicity. Methods: After internalization of A{beta}(42) into human neuroblastoma (SH-EP) cells, they were irradiated with moderately intense 670-nm laser light (1000 Wm(-2)) and/or treated with epigallocatechin gallate (EGCG). Results: In irradiated cells, A{beta}(42) aggregate amounts were significantly lower than in nonirradiated cells. Likewise, in EGCG-treated cells, A{beta}(42) aggregate amounts were significantly lower than in non-EGCG-treated cells. Except for the cells simultaneously laden with A{beta}(42) and EGCG, there was a significant increase in cell numbers in response to laser irradiation. EGCG alone had no effect on cell proliferation. Laser irradiation significantly increased ATP levels in A{beta}(42)-free cells, when compared to nonirradiated cells. Laser-induced clearance of Aβ(42) aggregates occurred at the expense of cellular ATP. Conclusions: Irradiation with moderate levels of 670-nm light and EGCG supplementation complementarily reduces A{beta} aggregates in SH-EP cells. Transcranial penetration of moderate levels of red to near-infrared (NIR) light has already been amply exploited in the treatment of patients with acute stroke; the blood-brain barrier (BBB) penetration of EGCG has been demonstrated in animals. We hope that our approach will inspire a practical therapy for AD. |
| Keywords: | Adenosine Triphosphate, Alzheimer Disease, Amyloid, Antineoplastic Agents, Brain Neoplasms, Catechin, Cultured Tumor Cells, Fluorescence Microscopy, Low-Level Laser Therapy, Neuroblastoma |
| Source: | Photomedicine and Laser Surgery |
| ISSN: | 1549-5418 |
| Publisher: | Mary Ann Liebert |
| Volume: | 30 |
| Number: | 1 |
| Page Range: | 54-60 |
| Date: | 4 January 2012 |
| Official Publication: | https://doi.org/10.1089/pho.2011.3073 |
| PubMed: | View item in PubMed |
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