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| Item Type: | Article |
|---|---|
| Title: | miR-184 regulates pancreatic β-cell function according to glucose metabolism |
| Creators Name: | Tattikota, S.G., Rathjen, T., Hausser, J., Khedkar, A., Kabra, U.D., Pandey, V.K., Sury, M.D., Wessels, H.H., Mollet, I.G., Eliasson, L., Selbach, M., Zinzen, R.P., Zavolan, M., Kadener, S., Tschöp, M., Jastroch, M., Friedländer, M.R. and Poy, M.N. |
| Abstract: | In response to fasting or hyperglycemia, the pancreatic {beta}-cell alters its output of secreted insulin; however the pathways governing this adaptive response are not entirely established. While the precise role of microRNAs (miRNAs) is also unclear, a recurring theme emphasizes their function in cellular stress responses. We recently showed that miR-184, an abundant miRNA in the {beta}-cell, regulates compensatory proliferation and secretion during insulin resistance. Consistent with previous studies showing miR-184 suppresses insulin release, expression of this miRNA was increased in islets after fasting, demonstrating an active role in the {beta}-cell as glucose levels lower and the insulin demand ceases. Additionally, miR-184 was negatively regulated upon administration of a sucrose-rich diet in Drosophila demonstrating strong conservation of this pathway through evolution. Furthermore, miR-184 and its target Argonaute2 (Ago2) remained inversely correlated as concentrations of extracellular glucose increased, underlining a functional relationship between this miRNA and its targets. Lastly, restoration of Ago2 in the presence of miR-184 rescued suppression of miR-375-targeted genes suggesting these genes act in a coordinated manner during changes in the metabolic context. Together, these results highlight the adaptive role of miR-184 according to glucose metabolism and suggest the regulatory role of this miRNA in energy homeostasis is highly conserved. |
| Keywords: | Glucose Metabolism, microRNA (miRNA), beta-Cell, Argonaute, Pancreatic Islet, Insulin, Insulin Secretion, microRNA Mechanism, Animals, Mice |
| Source: | Journal of Biological Chemistry |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Volume: | 290 |
| Number: | 33 |
| Page Range: | 20284-20294 |
| Date: | 14 August 2015 |
| Official Publication: | https://doi.org/10.1074/jbc.M115.658625 |
| PubMed: | View item in PubMed |
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