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| Item Type: | Article |
|---|---|
| Title: | S1P signalling differentially affects migration of peritoneal B cell populations in vitro and influences the production of intestinal IgA in vivo |
| Creators Name: | Kleinwort, A., Luehrs, F., Heidecke, C.D., Lipp, M. and Schulze, T. |
| Abstract: | Introduction: Sphingosine-1-phosphate (S1P) regulates the migration of follicular B cells (B2 cells) and directs the positioning of Marginal zone B cells (MZ B cells) within the spleen. The function of S1P signalling in the third B cell lineage, B1 B cells, mainly present in the pleural and peritoneal cavity, has not yet been determined. Methods: S1P receptor expression was analysed in peritoneal B cells by real-time polymerase chain reaction (qPCR). The chemotactic response to S1P was studied in vitro. The role of S1P signalling was further explored in a s1p(₄)(-/-) mouse strain. Results: Peritoneal B cells expressed considerable amounts of the S1P receptors 1 and 4 (S1P(₁) and S1P(₄), respectively). S1P(₁) showed differential expression between the distinct peritoneal B cell lineages. While B2 cells showed no chemotactic response to S1P, B1 B cells showed a migration response to S1P. s1p(₄)(-/-) mice displayed significant alterations in the composition of peritoneal B cell populations, as well as a significant reduction of mucosal immunoglobulin A (IgA) in the gut. Discussion: S1P signalling influences peritoneal B1 B cell migration. S1P(₄) deficiency alters the composition of peritoneal B cell populations and reduces secretory IgA levels. These findings suggest that S1P signalling may be a target to modulate B cell function in inflammatory intestinal pathologies. |
| Keywords: | Peritoneal B Cells, B1 B Cells, Sphingosine-1-Phosphate (S1P), Mucosal Immunity, Lymphocyte Trafficking, Animals, Mice |
| Source: | International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| Publisher: | MDPI |
| Volume: | 19 |
| Number: | 2 |
| Page Range: | 391 |
| Date: | 29 January 2018 |
| Official Publication: | https://doi.org/10.3390/ijms19020391 |
| PubMed: | View item in PubMed |
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