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| Item Type: | Article |
|---|---|
| Title: | Myb and NF-M: combinatorial activators of myeloid genes in heterologous cell types |
| Creators Name: | Ness, S.A., Kowenz-Leutz, E., Casini, T., Graf, T. and Leutz, A. |
| Abstract: | The c-Myb transcription factor regulates the differentiation of immature erythroid, lymphoid, and myeloid cells, although only the latter cells become transformed by the v-myb oncogene. These are also the only cells that express the Myb-regulated gene mim-1, suggesting that Myb requires tissue-specific, cooperating factors to activate such genes. Here, we investigated the tissue-specific regulation of the mim-1 promoter and found that it not only contains binding sites for Myb but also for NF-M, a myeloid-specific transcription factor that probably corresponds to mammalian C/EBP beta. Both types of binding sites were found to be required for full activity of the promoter. Remarkably, ectopic coexpression of Myb and NF-M proteins in erythroid cells or fibroblasts was sufficient to induce endogenous markers of myeloid differentiation, like the mim-1 and lysozyme genes. Our results indicate that c-Myb and NF-M proteins act as a bipartite, combinatorial signal that regulates the expression of myeloid-specific genes, even in heterologous cell types. |
| Keywords: | Myb Oncogene, C/EBP-Like Factors, Hematopoiesis, Transcription Factors, Gene Expression, Animals, Chickens |
| Source: | Genes & Development |
| ISSN: | 0890-9369 |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Volume: | 7 |
| Number: | 5 |
| Page Range: | 749-759 |
| Date: | May 1993 |
| Official Publication: | https://doi.org/10.1101/gad.7.5.749 |
| PubMed: | View item in PubMed |
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