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| Item Type: | Article |
|---|---|
| Title: | C/EBP beta regulation of the tumor necrosis factor alpha gene |
| Creators Name: | Pope, R.M., Leutz, A. and Ness, S.A. |
| Abstract: | Activated macrophages contribute to chronic inflammation by the secretion of cytokines and proteinases. Tumor necrosis factor alpha (TNF alpha) is particularly important in this process because of its ability to regulate other inflammatory mediators in an autocrine and paracrine fashion. The mechanism(s) responsible for the cell type-specific regulation of TNF alpha is not known. We present data to show that the expression of TNF alpha is regulated by the transcription factor C/EBP beta (NF-IL6). C/EBP beta activated the TNF alpha gene promoter in cotransfection assays and bound to it at a site which failed to bind the closely related protein C/EBP alpha. Finally, a dominant-negative version of C/EBP beta blocked TNF alpha promoter activation in myeloid cells. Our results implicate C/EBP beta as an important regulator of TNF alpha by myelomonocytic cells. |
| Keywords: | Base Sequence, Binding Sites, CCAAT-Enhancer-Binding Proteins, Cell Line, DNA, DNA-Binding Proteins, Molecular Sequence Data, Monocytes, Mutation, Nuclear Proteins, Promoter Regions (Genetics), Recombinant Fusion Proteins, T-Lymphocytes, Tetradecanoylphorbol Acetate, Trans-Activation (Genetics), Transcription Factors, Transfection, Cultured Tumor Cells, Tumor Necrosis Factor-alpha |
| Source: | Journal of Clinical Investigation |
| ISSN: | 0021-9738 |
| Publisher: | American Society for Clinical Investigation |
| Volume: | 94 |
| Number: | 4 |
| Page Range: | 1449-1455 |
| Date: | October 1994 |
| Official Publication: | https://doi.org/10.1172/JCI117482 |
| PubMed: | View item in PubMed |
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