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| Item Type: | Article |
|---|---|
| Title: | Exogenous administration of gangliosides displaces GPI-anchored proteins from lipid microdomains in living cells |
| Creators Name: | Simons, M., Friedrichson, T., Schulz, J.B., Pitto, M., Masserini, M. and Kurzchalia, T.V. |
| Abstract: | Exogenous application of gangliosides to cells affects many cellular functions. We asked whether these effects could be attributed to the influence of gangliosides on the properties of sphingolipid-cholesterol microdomains on the plasma membrane, also termed rafts. The latter are envisaged as lateral assemblies of sphingolipids (including gangliosides), cholesterol, and a specific set of proteins. Rafts have been implicated in processes such as membrane trafficking, signal transduction, and cell adhesion. Recently, using a chemical cross-linking approach with Madin-Darby canine kidney (MDCK) cells permanently expressing a GPI-anchored form of growth hormone decay accelerating factor (GH-DAF) as a model system, we could show that GPI-anchored proteins are clustered in rafts in living cells. Moreover, this clustering was dependent on the level of cholesterol in the cell. Here we show that incubation of MDCK cells with gangliosides abolished subsequent chemical cross-linking of GH-DAF. Furthermore, insertion of gangliosides into the plasma membrane of MDCK GH-DAF cells renders GH-DAF soluble when subjected to extraction with Triton X-114 at 4 degrees C. Our data suggest that exogenous application of gangliosides displaces GPI-anchored proteins from sphingolipid-cholesterol microdomains in living cells. |
| Keywords: | Carrier Proteins, CD55 Antigens, Cell Line, Cell Surface Receptors, CHO Cells, Cross-Linking Reagents, Fluorescent Antibody Technique, G(M1) Ganglioside, Gangliosides, Glucosides, Glycosylphosphatidylinositols, GPI-Anchored Folate Receptors, Growth Hormone, Membrane Lipids, Membrane Proteins, Orthomyxoviridae, Succinimides, Animals, Cricetinae, Dogs |
| Source: | Molecular Biology of the Cell |
| ISSN: | 1059-1524 |
| Publisher: | American Society for Cell Biology |
| Volume: | 10 |
| Number: | 10 |
| Page Range: | 3187-3196 |
| Date: | October 1999 |
| Additional Information: | Copyright (c) 1999 by The American Society for Cell Biology |
| Official Publication: | http://www.molbiolcell.org/cgi/content/abstract/10/10/3187 |
| PubMed: | View item in PubMed |
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