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| Item Type: | Article |
|---|---|
| Title: | ER-Golgi traffic is a prerequisite for efficient ER degradation |
| Creators Name: | Taxis, C., Vogel, F. and Wolf, D.H. |
| Abstract: | Protein quality control is an essential function of the endoplasmic reticulum. Misfolded proteins unable to acquire their native conformation are retained in the endoplasmic reticulum, retro-translocated back into the cytosol, and degraded via the ubiquitin-proteasome system. We show that efficient degradation of soluble malfolded proteins in yeast requires a fully competent early secretory pathway. Mutations in proteins essential for ER-Golgi protein traffic severely inhibit ER degradation of the model substrate CPY*. We found ER localization of CPY* in WT cells, but no other specific organelle for ER degradation could be identified by electron microscopy studies. Because CPY* is degraded in COPI coat mutants, only a minor fraction of CPY* or of a proteinaceous factor required for degradation seems to enter the recycling pathway between ER and Golgi. Therefore, we propose that the disorganized structure of the ER and/or the mislocalization of Kar2p, observed in early secretory mutants, is responsible for the reduction in CPY* degradation. Further, we observed that mutations in proteins directly involved in degradation of malfolded proteins (Der1p, Der3/Hrd1p, and Hrd3p) lead to morphological changes of the endoplasmic reticulum and the Golgi, escape of CPY* into the secretory pathway and a slower maturation rate of wild-type CPY. |
| Keywords: | Cycloheximide, Endoplasmic Reticulum, Genotype, Golgi Apparatus, Kinetics, Plasmids, Protein Folding, Saccharomyces Cerevisiae Proteins, Second Messenger Systems |
| Source: | Molecular Biology of the Cell |
| ISSN: | 1059-1524 |
| Publisher: | American Society for Cell Biology |
| Volume: | 13 |
| Number: | 6 |
| Page Range: | 1806-1818 |
| Date: | 1 June 2002 |
| Additional Information: | Copyright (c) 2002 by The American Society for Cell Biology |
| Official Publication: | https://doi.org/10.1091/mbc.01-08-0399 |
| PubMed: | View item in PubMed |
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