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| Item Type: | Article |
|---|---|
| Title: | Global transcriptome analysis of murine embryonic stem cell-derived cardiomyocytes |
| Creators Name: | Doss, M.X., Winkler, J., Chen, S., Hippler-Altenburg, R., Sotiriadou, I., Halbach, M., Pfannkuche, K., Liang, H., Schulz, H., Hummel, O., Huebner, N., Rottscheidt, R., Hescheler, J. and Sachinidis, A. |
| Abstract: | BACKGROUND: Characterisation of gene expression signatures for cardiomyocytes derived from embryonic stem (ES) cells will help define their early biological processes. RESULTS: A transgenic a-myosin heavy chain (a-MHC) ES cell lineage was generated, expressing puromycin resistance and enhanced green fluorescent protein (EGFP) under the control of the a-MHC promoter. A puromycin-resistant, EGFP+, a-MHC+ cardiomyocyte population was isolated with over 92% purity. RNA was isolated after electrophysiological characterisation of the cardiomyocytes. Comprehensive transcriptome analysis of a-MHC+ cardiomyocytes in comparison to undifferentiated a-MHC ES cells and the control population from 15-day old embryoid bodies (EBs) led to the identification of 884 upregulated probe sets and 951 downregulated probe sets in a-MHC+ cardiomyocytes. A subset of upregulated genes encodes for cytoskeletal and voltage-dependent channel proteins, and proteins participating in aerobic energy metabolism. Interestingly, mitosis, apoptosis and Wnt signalling-associated genes were downregulated in the cardiomyocytes. In contrast, annotations for genes upregulated in the a-MHC+ cardiomyocytes are enriched for the gene ontology categories: enzyme-linked receptor protein signalling pathway (GO:0007167); protein kinase activity (GO:0004672); negative regulation of Wnt receptor signalling pathway (GO:0030178); regulation of cell size (O:0008361); as well as for the Biocarta p38 MAPK signalling pathway and KEGG calcium signalling pathway. CONCLUSION: The specific pattern of gene expression in the cardiomyocytes derived from ES cells reflects the biological, physiological and functional processes occurring in mature cardiomyocytes. Identification of cardiomyocyte-specific gene expression patterns and signalling pathways will contribute towards elucidating their roles during intact cardiac function. |
| Keywords: | Apoptosis, Cell Differentiation, Cell Proliferation, Cluster Analysis, Embryonic Stem Cells, Gene Expression Profiling, Gene Expression Regulation, Genetic Engineering, Cardiac Myocytes, Oligonucleotide Array Sequence Analysis, Messenger RNA, Ventricular Myosins, Animals, Mice |
| Source: | Genome Biology |
| ISSN: | 1465-6906 |
| Publisher: | BioMed Central |
| Volume: | 8 |
| Number: | 4 |
| Page Range: | R56 |
| Date: | 11 April 2007 |
| Official Publication: | https://doi.org/10.1186/gb-2007-8-4-r56 |
| PubMed: | View item in PubMed |
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