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| Item Type: | Article |
|---|---|
| Title: | Spatial and single-cell profiling of the metabolome, transcriptome and epigenome of the aging mouse liver |
| Creators Name: | Nikopoulou, C., Kleinenkuhnen, N., Parekh, S., Sandoval, T., Ziegenhain, C., Schneider, F., Giavalisco, P., Donahue, K.F., Vesting, A.J., Kirchner, M., Bozukova, M., Vossen, C., Altmüller, J., Wunderlich, T., Sandberg, R., Kondylis, V., Tresch, A. and Tessarz, P. |
| Abstract: | Tissues within an organism and even cell types within a tissue can age with different velocities. However, it is unclear whether cells of one type experience different aging trajectories within a tissue depending on their spatial location. Here, we used spatial transcriptomics in combination with single-cell ATAC-seq and RNA-seq, lipidomics and functional assays to address how cells in the male murine liver are affected by age-related changes in the microenvironment. Integration of the datasets revealed zonation-specific and age-related changes in metabolic states, the epigenome and transcriptome. The epigenome changed in a zonation-dependent manner and functionally, periportal hepatocytes were characterized by decreased mitochondrial fitness, whereas pericentral hepatocytes accumulated large lipid droplets. Together, we provide evidence that changing microenvironments within a tissue exert strong influences on their resident cells that can shape epigenetic, metabolic and phenotypic outputs. |
| Keywords: | Epigenome, Hepatocytes, Liver, Metabolome, Transcriptome / Genetics, Animals, Mice |
| Source: | Nature Aging |
| ISSN: | 2662-8465 |
| Publisher: | Nature Research |
| Volume: | 3 |
| Number: | 11 |
| Page Range: | 1430-1445 |
| Date: | November 2023 |
| Official Publication: | https://doi.org/10.1038/s43587-023-00513-y |
| PubMed: | View item in PubMed |
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